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1、2023-2024学年天津市高三（上）全真模拟英语试卷一、单选题（本大题共15小题，共15.0分）1. Annie Salmon，disabled，is attended throughout her school days by a nurse _ to guard her.（）A. toappointB. appointingC. appointedD. havingappointed2. DoyoumindifIgooutthisevening?Idontmindwhatyoudo_youletmeknowaheadoftime.A. eversinceB. incaseC. aslon

2、gasD. eventhough3. Not only can the environment of a country suffer from the sudden growth of tourism but the people also rapidly feel its _.（）A. benefitsB. contributionC. demandD. effects4. Our ancestors enjoyed the healing power of nature，and now scientists are starting to _.（）A. holdbackB. moveon

3、C. catchupD. getacross5. Emoji can help communication feel friendlier，and _ a serious note can be softened with an encouraging smile.（）A. evenB. stillC. alsoD. yet6. Are you feeling any better，Lucy？ Yes.The medicine the doctor gave me brought _ to my headache.（）A. safetyB. shelterC. reliefD. defense

4、7. The core of life education at school lies in guiding students to_manage relationships.（）A. slightlyB. originallyC. temporarilyD. properly8. I have been convinced that the print media are usually more _ and more reliable than television.（）A. preciousB. efficientC. relevantD. accurate9. In order to

5、 make her 45th birthday impressive，I intend to buy her a wonderful present，_that is not expensive but very meaningful.（）A. itB. oneC. theoneD. this10. He suddenly spotted an old friend across the room，so he pushed his way _ the crowd of people to get to her.（）A. beyondB. throughC. downD. against11. Can you have a meal served outside the cafe？ I dont think so，but _.（）A. itsnotyourbusinessB. itsoundslikeagoodideaC. IcouldntagreemoreD. dontjumptoconclusions12. Ethan is asking whether he must attend the meeting tomorrow.

11.ACC.合成酶是乙烯合成的关键酶,乙烯的合成会影响番茄的储藏和运输。下图为科学家利用ACC合成酶基因的反向连接构建载体,通过基因工程设计耐储转基因番茄的流程图。下列说法错误的是注:ampR为氢苄青素抗性基因:1et^k为四环素抗性基因。A.引物的特异性是能够从番茄DNA中获取ACC合成酶基因的关键B.反向连接的ACC合成酶基因合成的mFRNA通过与正常的ACC合成酶基因的mRNA互补,限制了细胞内乙烯的合成C.可以在培养基中加人氨苄青霉素和四环素,存活下来的细胞内则含有携带目的基因的质粒D.设计双酶切处理目的基因及载体的目的是为了更好地保证目的基因的反向连接

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